Journal of Natural Remedies

K. S. Patil , P. Majumder , R. R. Wadekar

Abstract

Objective: To study the effect of aqueous extract of leaves of Enhydra fluctuans on neutrophil phagocytic function. Methods: The different concentrations of (25,50,100μg/ml) of extract of leaves of Enhydra fluctuans was subjected to study its effect on different in-vitro methods of phagocytosis such as neutrophil locomotion, chemotaxis, immunostimulant activity of phagocytosis of killed Candida albicans and qualitative Nitro Blue Tetrazolium test using human neutrophils. Result: This preliminary study revealed that Enhydra fluctuans extract has stimulated chemotactic, phagocytic and intracellular killing potency of human neutrophils at the different concentration. Conclusion: From the results obtained it can be observed that the aqueous extract of Enhydra fluctuans leaves stimulates cell-mediated immune system by increasing neutrophil function phagocytic activity.

Keywords

Immonostimulant Activity, Enhydra fluctuans, Neutrophils, Phagocytosis

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K. S. Patil , R. Kenia , S. C. Chaturvedi

Abstract

Objective: To study the anti-ulcer activity of stem bark of Shorea tumbuggaia in pylorus ligation method. Materials and method: Petroleum ether (60-80°), chloroform, ethanol and aqueous extract of Shorea tumbuggaia stem bark were tested for various preliminary phytoconstituents and were screened for anti-ulcer activities in pylorus ligation method in albino rats. Doses for the different extracts were selected based on the results of acute toxicity studies in mice. The effect was assessed by ulcer index, total acidity, free acidity, pH of gastric contents, serum calcium levels and serum alkaline phosphatase levels in pyloric ligation method. Result: Lipids, sterols, triterpenoids, tannins and phenol were found to be present in various extracts of Shorea tumbuggaia stem barks. The ethanolic extract of stem bark of Shorea tumbuggaia significantly raised the pH of gastric contents. It lowered the free acidity, total acidity and ulcer index as compared to control. It significantly raised the serum calcium levels and decreased the serum alkaline phosphates levels. Conclusion: From the results obtained it can be observed that ethanolic extract of stem bark of Shorea tumbuggaia has significant anti-ulcer property.

Keywords

Shorea tumbuggaia, Anti-ulcer, Pyloric Ligation

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R. G. Mali , J. C. Hundiwale , R. S. Gavit , K. S. Patil , M. V. Kulkarni

Abstract

Obejective: To study the effect of hydroalcoholic extract of aerial parts of Achyranthes aspera on neutrophil phagocytic function . Methods: The different concentrations (25,50 and 100 mg / ml ) of extract of aerial parts of Achyranthes aspera was subjected to study its effect on different in vitro methods of phagocytosis such as neutrophil locomotion and chemotaxis, in vitro immunostumulant activity by phyagocytosis of killed Candida albicans and qualitative nitro blue tetrazolium test using human neutrophils. Results: This preliminary study revealed that Achyranthes aspera extract has stimulated chemotactic, phagocytic and intracellular killing potency of human neutrophils at the concentration range of 25 - 100 mg / ml. Conclusion: From the results obtained it can be observed that the hydroalcoholic extract of Achyranthes aspera stimulates cell-mediated immune system by increasing neutrophil phagocytic function.

Keywords

Achyranthes Aspera, Neutrophils, Phagocytosis, Chemotaxis

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K. S. Patil , P. D. Warke , S. C. Chaturvedi

Abstract

Objective: To study the hypoglycaemic properties of Ficus glomerata fruits in alloxan induced diabetic rats. Materials and methods: Petroleum ether, benzene, chloroform, ethanol and aqueous extracts of F. glomerata fruits were screened for hypoglycemic properties in alloxan-induced diabetic rats. The effect was assessed by blood glucose, serum cholesterol, serum urea and serum triglyceride levels. Oral doses of petroleum ether extracts of 250 mg/kg body weight produced significant lowering of blood sugar, serum cholesterol, serum urea and serum triglyceride levels in alloxan-induced diabetic treated rats. The hypoglycemic effects were compared with those of glibenclamide. Conclusion: The present study shows that petroleum ether extracts of fruits of F. glomerata produce better hypoglycemic actions compared to other extracts.

Keywords

Acute Toxicity, Alloxan, Ficus Glomerata, Hypoglycemic Activity, Moraceae

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K. S. Patil

Abstract

Objective: To screen the wound healing activity of different extracts of seed kernels of Caesalpinia crista Linn in excision, incision and dead space wound models in albino rats. Materials and methods : Seed kernels of Caesalpinia crista Linn was defatted completely with petroleum ether (60-80°C) and then marc was subjected to continuous extraction using alcohol. The concentrated alcoholic extract was subjected to fractionation by solvent ether, ethyl acetate butanone and butanol in succession. Petroleum ether extract, alcoholic extract and four fractions were tested for various preliminary phytoconstituents and screened for wound healing activity in excision, incision and dead space wound models in albino rats. Results: Ethyl acetate fraction of seed kernel of Caesalpinia crista Linn has shown better wound healing activity in incision, excision and dead space wound models as compared to alcoholic extract and solvent ether fraction. While petroleum ether extract, butanol fraction and butanone fraction has shown the least effective wound healing activity. Conclusion: From the result obtained it can be concluded that ethyl acetate fraction of seed kernel of Caesalpinia crista Linn has significant wound healing property.

Keywords

Caesalpinia crista Linn, Wound Healing, Soxhlet Extraction

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K. S. Patil , V. V. Chaudhari , Sachin Mahajan

Abstract

Objective: To study the effect of methanol extract of Ipomoea batatas ischolar_main on neutrophils phagocytic function. Methods: Different concentrations (10, 20,50,100,1000 μg/ml) of methanol extract of Ipomoea batatas ischolar_main was subjected to study its effect on different in vitro methods of phagocytosis such as neutrophils locomotion and chemotaxis test, in vitro immunostimulant activity by slide method and qualitative nitro blue tetrazolium test using human neutrophils. Result: The methanolic extract of Ipomoea batatas ischolar_mains had stimulated chemotactic, phagocytic and intracellular killing potency of human neutrophils at the concentration range of 10-100μg/ml. Conclusions: The methanolic extract of Ipomoea batatas ischolar_mains stimulates cell mediated immune system by increasing neutrophil phagocytic function.

Keywords

Ipomoea batatas, Immunostimulant Activity, Neutrophils, Phagocytosis

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K. S. Patil , S. D. Mandawgade

Abstract

Objective : To screen the wound healing activity of different extracts of leaves of Lawsonia alba on excision, incision wound models in albino rats. Materials and methods : Crude extracts of the leaves of Lawsonia alba obtained by successive solvent extraction with petroleum ether (40-60°C), chloroform, ethanol 95% and finally maceration with chloroform-water, were subjected for phytochemical investigation and were screened for wound healing properties in the excision and incision wound models in albino rats. The flavonoid, luteolin present in the leaves was isolated from ethanol extract by column chromatography. Results : Flavonoids, steroids, tannins and glycosides were found to be present in ethanol extract and other extracts of the leaves of Lawsonia alba. In excision wound parameters the ethanol extract promotes better wound healing (97.28%) compared to control and other extracts. In resutured incision wound models, ethanol extract and petroleum ether extract showed significant Breaking strength (p<0.001) and (p<0.01) respectively compared to control. Conclusion : From the results obtained, it can be observed that ethanol extract of leaves of Lawsonia alba have significant wound healing property. Also it can be concluded that flavonoid may be responsible for wound healing activity.

Keywords

Lawsonia alba, Flavonoids, Wound Healing, Successive Solvent Extraction

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K. S. Patil , Ganesh Wadkar , Sunil Mathapati , Maheshwar Hogade , Sunil Karale , Sunil Deshpande

Abstract

The purpose of the present study is to evaluate the anti-ulcer activity of Caesalpinia bonducella (Linn.) Flem. The methanolic extract of Caesalpinia bonducella (Linn.) Flem. leaves were evaluated for its anti-ulcer activity against aspirin plus pylorous ligation induced gastric ulcer (antisecretory) in rats, water immersion stress induced ulcer models in rats and HCl-Ethanol induced ulcer (cytoprotective) in mice at two different doses viz. 100 and 200 mg/ kg. Biochemical parameters like volume, pH, free acidity, total acidity of the gastric juice, ulcer index and percentage protection were studied. The methanolic extract of Caesalpinia bonducella leaves in aspirin plus pyrolus ligation showed significant (P<0.05, P<0.01) reduction in gastric volume, free acidity, total acidity and ulcer index at dose of 100 and 200 mg/kg as compared to control, there was marked increase in pH at dose of 100 and 200 mg/kg. The plant extract at both dose of 100 and 200 mg/kg showed significant gastro protective activity in water immersion stress induced ulcer and HCl-Ethanol induced ulcer. Thus, the present results indicate that the methanolic extract of Caesalpinia bonducella (Linn.) Flem. leaves have significant anti-ulcer activity.

Keywords

Caesalpinia bonducella, Anti-ulcer, Aspirin Pylorus Ligation Ulceration, HCL-ethanol Ulceration, Water Stress Ulcer

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